Build a pyramid in less than 60 seconds!
The Leica EM Trim2 is a high speed milling system with an integrated stereo microscope and LED ring illuminator for trimming of biological and industrial samples prior to ultramicrotomy. A pivot arm and adjustment assembly holds the specimen carrier for optimum orientation. It can be used with either tungsten carbide or diamond milling tools. The area of interest can be centered and a flat block face milled onto the front face of the sample.
For TEM and LM the sample must be trimmed to shape by adjusting the angle of the pivot arm and trimming the desired block shape - pyramidal, square rectangular, etc...
Viewing of the specimen perpendicular to the axis of the stereomicroscope allows distance definition i.e. from the front face of the sample. All this takes place under constant observation with the stereo microscope.
Key Features
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With accurate, parallel edges
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Withe the angle of the pyramid sides set to user-preferred angle up to 60°
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With smooth and clean surfaces
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Any part of the sample can be targeted
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With perpendicular viewing of the embedded sample to determine its position for accurate milling
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With a 1 µm step removal rate and an ergonomically positioned feed hand wheel
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In any desired size from approximately 0.2 mm square upwards
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With stereo microscope and ring LED illumination for clear observation during milling of the sample
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Safer than conventional razor blade trimming
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For rapid alignment of the vertical block face to the ultramicrotome knife with minimal adjustment
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With an adaptor for the cryoultramicrotome for pre-trimming samples at room temperature prior to trimming in the cryo-chamber (e.g. polymers)
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With a low-noise extraction filtration unit with Hepa filter for a safe and silent environment (optional)
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With fibre Optic transillumination system for locating small samples and translucent objects (optional)
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During operation the user is:
- Not in contact with dangerous components as the miller is located under a protective cover
- Safe, as the instrument will not run unless the cover is closed
- The spindle motor stops quickly as soon as the cover is opened
- To be easily accessible the miller ON/OFF switch is integrated into the control lever for the milling action
- Hazardous waste, resin dust and fragments which under razor blade trimming become airborne, are drawn into an extraction and filtration unit fitted with a Hepa-filter (optional).
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With a tungsten carbide or diamond milling tool, block faces of biological and industrial specimens can be trimmed prior sectioning with an ultramicrotome. A stereo microscope in conjunction with the ring LED illumination provides for brilliant observation during milling the sample. With the swivelling specimen arm the pyramided sides can be set up to 60°.
Three stop positions of the specimen arm provides for face side milling, frontal observation and 90°view rotation. Accurate sample removal is achieved with the 1µm step advance of the ergonomically positioned encoder hand wheel. An optional low-noise mobile extraction and filtration unit with a Hepa filter provides for silent and safe environment conditions.
Brilliant LED top light ring illumination with optimised back lighting provides excellent sample obervation during trimming. Additionally, the fibre optic transillumination system (optional) enables location of small samples and translucent objects
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